Reporter

Part:BBa_K4770013:Design

Designed by: Andrea Camí Bonet   Group: iGEM23_Barcelona-UB   (2023-10-11)


mCherry_Gene

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 349
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 349
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 349
  • 1000
    COMPATIBLE WITH RFC[1000]

Design considerations

MoClo kit provides you with sequences with different overhangs so you can choose the one that better fits you design/cloning plan. Sequence contains an intron (CrRbcS2 intron 1) for an intron-mediated enhancement (IME).

Source

Original sequence was obtained from MoClo kit designed by Crozet, et. al (2018).